Metabolome Analysis Facility

 

Overview of Services

Metabolic Function: The core offers targeted an non-targeted methods using liquid chromatography coupled with mass spectrometry systems to perform metabolic phenotyping of amino acids, free fatty acids, sphingolipid ceramides, lipoprotein ceramides, fatty acylcarnitines, phospholipids, and diacyglycerols.

Mitochondrial Phenotyping: The core provides support for mitochondrial phenotyping including purification of mitochondria and analysis of size and internal complexity, metabolic assays of respiration on tissue slices or cells using Seahorse assays, and other activities  

Leadership

John Hollander Ph.D.

Professor and Graduate Director, Exercise Physiology
Sr. Asst. Dean for Research and Graduate Education, Human Performance
Director, Mitochondria, Metabolism & Bioenergetics
West Virginia University School of Medicine
304 293-3683
jhollander@hsc.wvu.edu

 

Jim Simpkins Ph.D.

Professor

The Highland Chair of Stroke Neurology

Director, Center for Basic and Translational Stroke

304-293-7430

jwsimpkins@hsc.wvu.edu

 

 

Christopher F. Cuff Ph.D.

Director of Core Resources
304-293-4622
ccuff@hsc.wvu.edu

Location and hours of operation

Hours Location

M-F  
9 AM - 5 PM

Mass spectrometry: Rm 2033 Health Sciences Center North           

Mitochondria Phenotyping: Rm  3xxx Health Sciences Center North

Links and Resources

  1. Nexera UC - Supercritical Fluid Extraction/Chromatograph System
  2. QTRAP® 5500 LC-MS/MS System

Contacts

Name Role Phone Email Location
Terry McManus Ph.D.
Research Associate - Metabolomics
 
304-293-0494
 
tmcmanus@hsc.wvu.edu
 
2033 HSC-N
 
Mark Pinti
Research Assitant- Mitochondria Phenotyping
 

 
mpinti@mix.wvu.edu
 

 
Deborah
Research Assistant- Mitochondria Phenotyping
 

 

 

 

Service list


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Metabolomics Core Services (1)