Flow Cytometry and Single Cell Core

Overview of Services

The WVU Flow Cytometry & Single Cell Core Facility (FCSCCF) is a fee for service facility that provides instrumentation and scientific support for single cell analysis and sorting. The facility routinely performs analysis of both eurkaryotic and prokaryotic cells for expression of intracellular and extracellular proteins, cell cycle quantificatikon, cytokine production, and cell sorting based on expression of cell surface antigen(s) and/or expression of genetically engineered intracellular fluorescent proteins.

The facility is equipped with two cytometers: a 4 laser BD FACSAria high speed bench top sorter, and a 4 laser BD LSRFortessa with a high throughput sampling module and a detector to analzye submicron size particles. For small particular detection (10 - 2000 nm), the facility has a NanoSight NS300 that detects, counts, sizes and determines, using fluorescently labeled antibodies, 1 - 4 proteins expressed by the particles. The facility also has a Fluidigm C1 Single Cell Auto Prep System, which uses an integrated fluidic circuit chip to isolate 96 individual cells, extract the RNA/DNA/microRNA from each cell, amplify the RNA and make cDNA. The resulting products can be sequenced or used in PCR.


Additional instrumentation in the lab includes:

 Miltenyi Biotec gentleMACS Otto Dissociator
For making single cell suspensions or homgenates from tissues

Miltenyi Biotec AutoMACS
For sterile magnetic cell sorting

Zetasizer Nano Z
To measure the mobility and zeta potential of dispersed particles and molecules in solution

Meso Scale Discovery Sector 2400
Automated plate reader that detects multiple analytes by electrochemiluminiscence

Leadership

Kathleen Brundage, Ph.D.
Director of WVU Flow Cytometry & Single Cell Core Facility
Research Assistant Professor
West Virginia University
kbrundage@wvu.edu
(304) 293-6273

Location and hours of operation

Hours Location

Staffed Hours

 

9 AM - 5 PM

 HSC North Room 2160

Links and Resources

  1. http://flowcore.hsc.wvu.edu

Contacts

Name Role Phone Email Location
Kathleen Brundage
Core Director
 
(304) 293-6273
 
kbrundage@wvu.edu
 
HSC North Room 2160a
 

Service list


Search available services: View: by category alphabetically
unclassified (1)