Flow Cytometry and Single Cell Core

Overview of Services

The WVU Flow Cytometry & Single Cell Core Facility (FCSCCF) is a fee for service facility that provides instrumentation and scientific support for single cell analysis and sorting. The facility routinely performs analysis of both eukaryotic and prokaryotic cells for expression of intracellular and extracellular proteins, cell cycle quantification, cytokine production, and cell sorting based on expression of cell surface antigen(s) and/or expression of genetically engineered intracellular fluorescent proteins.

The facility is equipped with three cytometers: a 4 laser BD FACSAria high speed bench top sorter equipped with an automated cell deposition unit (ACDU) for single cell sorting into plates, a 4 laser BD LSRFortessa with a high throughput sampling module and a detector to analyze submicron size particle, and a 3 laser Cytek Aurora full spectrum analyzer with a 96 well plate loader. For small particle detection (10 - 1000 nm), the facility has a NanoSight NS300 that detects, counts, sizes, and determines, using fluorescently labeled antibodies, 1 - 4 proteins expressed by the particles.

For single cell RNAseq, ATACseq and spatial transcriptomic analysis, the facility has a 10x Genomic Chromium X and 10x Genomics CytAssist. The 10x Genomics Chromium X barcodes individual cell’s RNA for bulk single cell RNAseq and multiomic analysis including gene expression, cell surface proteins, antigen specificity, and chromatin accessibility. Samples can be either fixed or live cells, up to 20,000 cells can be loaded at once, and on-chip multiplexing can be done. The 10x Genomics CytAssist allows for the mapping of the transcriptome to FFPE, Fresh Frozen, and Fixed Frozen tissue sections. 

Additional instrumentation in the lab includes:

Luminex MAGPIX
Imaging-based analyzer that simultaneously measures up to 50 analytes in one well

Meso Scale Discovery QuickPlex SQ120
Automated plate reader that detects multiple analytes by electrochemiluminescence

 Miltenyi Biotec gentleMACS Otto Dissociator
For making single cell suspensions or homogenates from tissues

Miltenyi Biotec AutoMACS Pro
For sterile magnetic cell sorting

Zetasizer Nano Z
To measure the mobility and zeta potential of dispersed particles and molecules in solution

Personnel

Location and hours of operation

Links and Resources

1. http://flowcore.hsc.wvu.edu

Contacts